NucleoGene 2X Hot Start PCR Master Mix is a ready-to-use reaction mixture for routine PCR amplification of 2X concentrate, 4 kb DNA fragments, and then PCR products are analyzed by loading on agarose gel. It contains all components, except primers and DNA or RNA template. NucleoGene 2X Hot Start PCR Master Mix simplifies the preparation of the reaction, increases the reproducibility of the test and reduces the risk of contamination. Taq DNA polymerase in the master mixture is inactivated with monoclonal antibodies which bind the polymerase and keep it inactive before the first PCR denaturation step. After heat activation, the antibodies are completely adjusted to release Taq DNA polymerase.
This serves to prepare the reaction mixture at room temperature, and to provide specific and efficient primer elongation. Thanks to its unique blend, it is resistant to inhibitors from the samples in your PCR applications. So that you can implement a PCR application without problems. Also suitable for Multiplex PCR applications.
Lane 1, 2, 3, 4 and 5 respectively; PCR results of the GAPDH gene with DNA isolated from formalin-fixed paraffin embedded kidney, liver, brain, heart and stomach tissues.