It allows you to make the best hotstart PCR applications with taq polymerase combined with an chemical modification. The special additives in its content help to eliminate foaming of the mixture resulting from vortexing, so that valuable samples are retained with fewer errors. Specially developed buffer chemistry works in harmony with almost all of your reaction designs. NucleoGene 2X qPCR Probe Master Mix reagents contain an inhibitor neutralizing additive that inhibits several types of PCR inhibition from clinical, plant, or environmental samples or complex food matrices, providing reliable qPCR assay performance. So you can do all the environmental analysis, GMO (genetically modified organism) analysis, analysis for clinical samples (stool including). In addition, NucleoGene 2X qPCR Probe Master Mix reagents are optimized for use in single-step RNA analyzes and different RNA analyzes.
- Extreme thermal stability: withstands three months at 25 oC with no impact on efficiency
- Chemical based hot start system reduces non-specific amplification and primer dimers
- Overcomes inhibition in crude extracts and environmental samples
- Amplifies up to 4 kb from genomic DNA, and GC-rich targets with up to 70% GC-content
- License-free for commercial or diagnostic use
Stable at 50 oC for 8 days, or 25 oC for 3 months, NucleoGene mixes ship economically at ambient temperatures, prevent Cq drift over time due to thermal degradation, and are ideal for high throughput or field applications where temperature control is not always ideal.
NucleoGene 2X qPCR Probe Master Mix has been validated against genomic, plasmid, and cDNA targets from a wide range of organisms. The mix supports both standard and fast protocols, and amplifies GC-rich targets up to 70%.
Numerous enhancers bind & inactivate PCR inhibitors, allowing amplification from crude extracts of:
- Food & beverages
- Plant & animal tissue
- Environmental air & water samples
Multiplex qPCR often suffers from competitive inhibition, where a high copy number target amplifies early, consuming reactants that prevent lower copy number targets from amplifying. With high levels of Taq, dNTPs, and multiplexing enhancers, NucleoGene 2X qPCR Probe Master Mix has been demonstrated to amplify four multiplex targets simultaneously, with no change in Cq when compared with singleplex reactions.
|Hot Start Mechanism||Chemical|
|GC Content||30 – 70%|
|Multiplex Targets||Up to 4|
|Exonuclease Activity||5' → 3'|
|Supported Probes||TaqMan/Hydrolysis, Molecular Beacon, Scorpions|
|Supported Templates||Genomic DNA, cDNA, Plasmid DNA|
|Recommended Reaction Volume||10 – 50 μL|
|High Temperature Stability||8 days @ 50 oC, 3 months @ 25 oC|
|Freeze/Thaw Cycles||Up to 20|
|Shipping Conditions||Gel Ice|
|Storage Conditions||-20 oC 1 year, or 4 oC for 6 months. Protect from light|
The mix ships with an optional ROX reference dye, allowing it to be used with high, low, and no- ROX qPCR instruments.