NucleoGene RNA to cDNA Mix (cDNA Synthesis Kit) (5X) 1

NucleoGene RNA to cDNA Mix (cDNA Synthesis Kit) (5X)

NucleoGene cDNA Synthesis Kit provides a rapid and sensitive method for first-strand cDNA synthesis, which displays excellent linearity across a wide range of starting material. This gives the same relative representation in cDNA templates, regardless of gene abundance, making it excellent for use in qPCR studies.
NucleoGene cDNA Synthesis Kit contains a highly-pure reverse transcriptase and optimized buffer system, which includes a unique blend of random hexamers and anchored oligo (dT) primers to deliver the highest quality qPCR ready cDNA. This makes the NucleoGene cDNA Synthesis Kit ideal for working with limited sample volumes, such as micro dissected samples and tissue biopsies (down to 1 pg of input RNA), to reverse transcribe precious RNA into stable cDNA ready for accurate real-time quantification. The unique blend of random hexamer primers and anchored oligo dT in the Buffer also ensure unbiased 3’ and 5’ coverage and reverse transcription of all regions.

NucleoGene cDNA Synthesis Kit can be used with Probe and SYBR® Kits for real-time RT-qPCR without compromising on quality, giving real-time results.

- Efficient – high-target affinity, coupled with a buffer system for improved yield of full-length cDNA
- Unbiased – optimized mix of random hexamers and anchored oligo dT primers for complete 5’ to
3’ RNA sequence representation
- Sensitive – lower Ct values from a broad range of input cDNA concentrations, enabling accurate
detection of very low-copy targets
- Robust – reliable reverse transcription under challenging conditions, including complex templates
and in the presence of inhibitors

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